DIBMA Glucosamine, HEPES

With diisobutylene-maleic acid (DIBMA) you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS that would usually interfere with the proteins function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm in comparison to SMAs, as their aromatic amino acids would usually absorb at the same spectrum and therefore interfere with protein quantification. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations.

One challenge when working with traditional DIBMA is its sensitivity to the presence of ions inside the buffer / media. The reason for this is DIBMAs own charge that lets it interact with these ions. This leads to precipitation of DIBMA and therefore loss of its function. To overcome this issue we modified DIBMA to have a reduced charge to drastically reduce the affinity to the ions. This was achieved by adding Glucosamine or an amino-functionalized diol to DIBMA. This modified DIBMA can be used for experiments for which the presence of ions is crucial for their success.

Features

Usage: Protein solubilization

Formula Weight: ~10,000 or 12,000 g/mol

pH: 7.5 in buffer

dn/dc: 1.35 M-1

Solubility: > 10 % in H2O

Absorbance at 280 nm: < 0.3 (1 % solution)

Mg2+ Tolerance: Depends on DIBMA product (Increased with less charged DIBMAs)

Ca2+ Tolerance: Depends on DIBMA product (Increased with less charged DIBMAs)

Shipping Temperature: ambient temperature

Storage of lyophilized copolymer: -20°C for several years

Storage of dissolved copolymer: 2-8°C for several days