Description
PureCube Fe-NTA MagBeads were developed to enrich phosphorylated proteins. They can also be used to purify his-tagged proteins as a secondary function. The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Fe-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding a high degree of reproducibility.
Features
Usage: Enrichment of phosphopeptides. Specific binding and purification of 6x his-tagged proteins
Specificity: Affinity to His-tagged proteins. Affinity to phosphorylated biomolecules
Bead ligand: Fe-NTA
Bead size: 30 µm
Filling quantity: Delivered as a 25 % suspension
Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability: 2-14
Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
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For research use only. Not for diagnostic or clinical use.