Description
PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 40 µm and thus great mechanical stability.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, NTA agarose resin is offered uncoupled to any metal ion. Through the coupling protocol, you can in turn attach your desired metal ions to the agarose beads.
Features
Usage: For coupling of metal ions for corresponding purification and / or enrichment purposes
Specificity: Varies
Binding capacity: Depends on the loaded ion
Purity: Depends on the loaded ion
Bead Ligand: NTA
Bead size: 40 μm
Matrix: 7.5% cross-linked agarose
Filling quantity: Delivered as a 50 % suspension
Recommended FPLC flow rates: 0.5-2 ml/min
Maximal FPLC flow rate: 6 ml/min
pH stability: 2-14
Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
For research use only. Not for diagnostic or clinical use.