Description
Alpha-glia Expansion Medium is a complete medium designed to promote optimal growth of normal human brain-derived microglia in vitro. It is a sterile, liquid medium which contains:
- Essential and non-essential amino acids
- Vitamins
- Organic and inorganic compounds
- Hormones
- Growth factors
- Trace minerals
- Fetal bovine serum (5%).
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human microglia in vitro.
Alpha-glia Expansion Medium consists of 445 ml of basal medium, 25 ml of fetal bovine serum 25 ml of microglia growth supplement and 5 ml of penicillin/streptomycin solution.
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For research use only. Not for diagnostic or clinical use.