pNF-H Sandwich ELISA Kit

ELISA kit for sensitive detection of Phosphorylated Neurofilament-H (pNF-H). Expression of this protein is up regulated in a variety of damage and disease states, and can be used to accurately quantify this up regulation. The kit can also detect pNF-H in the sera and spinal cord fluid (CSF) of animals with spinal cord and brain lesions. This protein is not normally found in sera or CSF, so its presence indicates recent axonal injury as a result of either damage or disease. This suggests pNF-H is a useful biomarker of neuronal and more specifically axonal injury or degeneration, a suggestion supported by a growing list of basic science publications referencing use of this kit. (e.g. Shaw et al. 2005, Lewis et al. 2008, Boylan et al. 2009, Lewis et al. 2010

• 96 well plate coated with the chicken antibody which has been blocked and so is ready to use.-he plate can be separated into 8 12 well strips.
• NF-H detection antibody and a pure pNF-H standard.
• Goat anti-rabbit alkaline phosphatase conjugate, p-Nitrophenol phosphatase substrate and buffer concentrates.
• Detailed Protocol.