AASTY 6-55

AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-55 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-55 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-55 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question peforms differently well with each polymer.

Features

State: Lyophilized Powder, to be solved with water

Molecular Weight: 5-6 kDa

Buffer: HEPES

pH after solving: 7.5

Divalent cationic tolerance: 6 mM

Acrylic Acid to Styrene Ratio: 55% to 45%

Shipping Temperature: ambient temperature

Storage of lyophilized copolymer: -20°C for several years

Storage of dissolved copolymer: 2-8°C for several days