Glyco-DIBMA

Glyco-DIBMA is a modified version of DIBMA with an added sugar side-chain to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose.

DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before.

Features

State: Lyophilized Powder, to be solved with water

Molecular weight: 15701 g/Mol

pH after solving: 7.5

Solubility: >10%

Divalent cationic tolerance: < 4 mM

Adsorbance (280 nm, 1% solution): > 0.3

Buffer: HEPES

Specific gravity: 1.1

Shipping Temperature: ambient temperature

Storage of lyophilized copolymer: -20°C for several years

Storage of dissolved copolymer: 2-8°C for several days