Sulfo-DIBMA

Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA) you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs in comparison usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.

Another major advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.

Features

Usage: Membrane protein solubilization & stabilization

Full-Name: Poly(diisobutylene-alt-N´,N´-/dimethyl(maleimidopropyl))ammonium propane sulfonate copolymer, sodium salt in 50 mM HEPES, pH 7.5

Formula Weight: 8,000 g/mol ± 1000 g/mol

Buffer: 50 mM HEPES

pH upon addition of dH2O: 7.5 ± 0.3

Total pH stability: 4-10

State: Lyophilized Powder

Recommended concentration range upon use: 1% to 5%

Solubility: > 10 % in H2O

Absorbance at 280 nm: < 0.3 (1 % solution)

Mg2+ Tolerance: over 100 mM

Ca2+ Tolerance: over 100 mM

Color: White to slightly yellow

Odor: Odorless

Shipping Temperature: ambient temperature

Long-term Storage (lyophilized copolymer): -20°C for several years

Short-term Storage (dissolved copolymer): 2-8°C for several days