Sulfo-Cubipol Lite

The proprietary Cubipol backbone, developed in-house by Cube Biotech, condenses the positive effects of previously available backbones like SMA, DIBMA, and Ultrasolute™ Amphipol. This new development delivers a new multimodal copolymer backbone, making the Cubipol series the first commercially available next-generation copolymer with 8 available off-the-shelf modified variants.

It joins the Next Generation Copolymers AASTY and Ultrasolute™ Amphipol, making solubilization and stabilization of membrane proteins a simpler and more effective endeavor than ever before.

Screening is key to discovering the best copolymer to stabilize your membrane protein target.

The low charged, zwitterionic copolymer variation resembles softer surfactants with a high resistance to extreme pH values, ionic strength and divalent ion concentrations (details in the table below). It belongs to the Cubipol class with low charge, which allows for the uninterrupted study of charge-sensitive interactions between membrane proteins and lipids.

The Sulfo-Cubipol series offers three versions—Lite, Medium, and Standard—each with varying levels of sulfobetaine functionalization to enhance hydrophilicity. Adjusting these properties optimizes stabilization and purification of membrane proteins for sensitive applications like cryo-TEM.

Features

State: Lyophilized Powder, to be solved with water

Full Name: Sulfo-Cubipol Lite

Buffer: HEPES

pH after solving: 7.5

pH Stability of Copolymer: 5.5-10

Absorbance at 280nm: No

Molecular Weight: 9 kDA

Ca2+ tolerance: 40 mM

Mg2+ tolerance: 40 mM

Solubility: > 10% (H2O)

Polymerization Type: Free-Radical

Shipping Temperature: Ambient Temparature

Storage of lyophilized copolymer: -20°C for several years

Storage of dissolved copolymer: 2-8°C for several days