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Neuromics

Protein Purification

Strep-Tactin®XT 4Flow® high capacity resin

Applications
Protein Purification

Description

Replacing:

Name

Catalog Number

Strep-Tactin®XT Superflow® High Capacity Resin

2-4030-002

Advantages of Strep-Tactin®XT 4Flow® vs. Strep-Tactin®XT Superflow®:

 

Strep-Tactin®XT 4Flow®

Strep-Tactin®XT Superflow®

pH stability

4-10

6-10

Protein binding capacity

High

High

Addressed protein size

Small to large proteins

Small to medium proteins (< 90 kDa)

Yield of low abundant proteins

Excellent

Excellent

Yield of large proteins

5-fold increased

Acceptable

Compatible with challenging proteins

Perfectly suitable

Perfectly suitable

Strep-Tactin®XT 4Flow® high capacity is used for purification of recombinant Strep-tag®II and Twin-Strep-tag® fusion proteins under gravity flow and low pressure or FPLC.

The Strep-Tactin®XT 4Flow® high capacity resin provides 2 to 3 times higher binding capacity for Strep-tag® proteins than standard Strep-Tactin®XT 4Flow® and therefore, higher yields of highly pure Strep-tag® protein.

Dynamic binding capacity: 16 mg protein/ml resin, determined with mCherry-Twin-Strep-tag® (30kDa).

Strep-Tactin®XT 4Flow® high capacity resin
Strep-Tactin®XT 4Flow® high capacity resin

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For research use only. Not for diagnostic or clinical use.