SARS-CoV-2 Spike Protein in LMNG Detergent

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers this intact form of SPIKE. Figure 1 highlights how this stabilization is achieved and provides insights into the overall structure of SPIKE.

Features

Alternative names: SPIKE_SARS2 Spike glycoprotein

UniProt Number: P0DTC2

Protein class: Single span transmembrane protein

Organism: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Sequence: Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence

Affinity tags: C-terminal Rho1D4-tag

Expression Host: Hek293 Expi cells

Size: 1286 amino acids (including Rho1D4-tag and linker), 142114 Da

Buffer composition: 20 mM Hepes pH 7.5; 150 mM NaCl, 0.01 % LMNG

Purity: >98%, determined via SDS-PAGE (Figure 2 A and B)

Homogeneity: Native Gel

Purified via: PureCube Rho1D4 Agarose

Function: Host cell surface receptor binding; fusion of virus membrane with host endosome membrane

Shipping conditions: Dry Ice

Storage conditions: –80°C. Avoid freeze-thaw cycles