Description
The discovery of Green Fluorescent Protein (GFP) is one of the most influential proteins in modern biology. Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection. Purified eGFP can find multiple uses in scientific research. Often as positive controls for protein purifications or western blots. Therefore we offer eGFP tagged with the most commonly used affinity tags for these purposes.
Features
Protein class: Beta-barrel; Autofluorescent Protein
Sequence - Sections named from N-Ter to C-Tery: GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP Spacer, DYKDDDDK-tag
Affinity tags: GST-tag (N-Terminus), Rho1D4-tag (C-terminus)
Size (kDA): 55.5 kDA
Size (Amino Acid count): 486
Excitation maximum: 488 nm
Fluorescence maximum: 509 nm
Purity: >98% as determined by SDS-PAGE
Related products
For research use only. Not for diagnostic or clinical use.