PureCube Ni-NTA Agarose

PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography. Their size range from market standard 40 µm and 100 µm diameters to 400 µm (XL).

These three options provide plenty of possibilities to choose the correct agarose resin for your purpose. Chose smaller beads for higher mechanical stability and higher protein yield. Meanwhile bigger beads provide faster flow rates and the possibility to work with very viscous cell media.

Features

Usage: Specific binding and purification of 6x His tagged proteins

Specificity: Affinity to His tagged proteins

Binding capacity: 20 - 80 mg/ml, depending on bead size

Bead Ligand: Ni-NTA

Bead size: 40 μm, 100 µm, or 400 µm

Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA

Filling quantity: Delivered as a 50 % suspension