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Neuromics

Protein Purifications

PureCube HiCap StrepTactin Agarose

Applications
Protein Purification

Description

PureCube Strep-Tactin® agarose resin beads consist of 6% cross-linked agarose that is coupled to the Strep-Tactin® protein. They enable protein yields of up to 5 mg protein per ml resin. They were developed for FPLC and batch spin-based protein purification.

Features

Usage: Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins

Specificity: Affinity to Strep-tagged (WSHPQFEK) proteins

Binding capacity: >5 mg/ml

Bead Ligand: Strep-Tactin®

Bead size: 100 μm

Matrix: 6% cross-linked agarose

Recommended flow rates: 0.25 - 1 ml/min

Max. flow rates: 6 ml/min

Filling quantity: Delivered as a 50 % suspension

Chemical stability: 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

Short-term storage: In neutral buffer at 4°C

Long-term storage: In neutral buffer with 20% ethanol at 4°C

PureCube HiCap StrepTactin Agarose

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For research use only. Not for diagnostic or clinical use.