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Neuromics
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Primary Antibody · Chicken IgY

Cas9 from Staphylococcus aureus

Species
Not Applicable
Applications
ICC, WB, IHC, IF

Description

The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has changed the field of gene editing. These repeated sequences are found in bacterial genomes with short DNA sequences derived from viruses which have infected the bacteria interspaced. These virally derived sequences can make short RNA sequences which can hybridize with specific viral DNA and target a nuclease, such as Cas9, to the viral sequence. So, if the bacteria are infected by this virus again, Cas9 can be directed to cleave the specific viral sequence and so inactivate the virus. By careful design of the RNA sequence the system can be used to specifically cut DNA virtually anywhere, including in living human and other mammalian cells. 

Our antibody is a polyclonal raised in chicken against the C-terminal 251 amino acids of of the Staphylococcus aureus protein and binds this protein transfected into cells, on western blots and in immunocytochemistry. The homologous region of the S. pyogenes is not closely related in amino acid sequence and, as expected, this antibody does not recognize that protein
Transfected HEK293 cells which overexpressing GFP-Cas9-SA fusion protein were stained with CH22120. Cells which are transfected with GFP-Cas9 are bright green (left). Staining with CH22120 is shown in red (middle). Merged image (right), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.
Transfected HEK293 cells which overexpressing GFP-Cas9-SA fusion protein were stained with CH22120. Cells which are transfected with GFP-Cas9 are bright green (left). Staining with CH22120 is shown in red (middle). Merged image (right), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.

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For research use only. Not for diagnostic or clinical use.