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Neuromics
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Human cells · Primary Cells

GFP Expressing Human Umbilical Vein Endothelial Cells

Cited in 4 papers
Species
Human
Applications
Cell Assays

Published with this product (4)

Description

GFP expressing human umbilical vein endothelial cells were isolated from normal human umbilical vein and transfected with GFP-lentiviral particles at passage 1. Puromycin resistant GFP-HUVECs are passage 3 and are shipped frozen (or in proliferating culture with a confluency of >90 %, GF01-T25). ENDO-Growth Media (MED001) is recommended for culture. Cells have an average additional population doubling levels >18 when cultured. 

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.

Product Highlights:
GFP-HUVECs stained with VE-Cadherin and DAPI on an egg white based hydrogel.
GFP-HUVECs stained with VE-Cadherin and DAPI on an egg white based hydrogel.
IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.
IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.
Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.
Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.

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For research use only. Not for diagnostic or clinical use.