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Neuromics
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Human cells · Primary Neurons

GFP Expressing Human Umbilical Vein Endothelial Cells

Species
Human
Applications
Cell Assays

Description

GFP expressing human umbilical vein endothelial cells were isolated from normal human umbilical vein and transfected with GFP-lentiviral particles at passage 1. Puromycin resistant GFP-HUVECs are passage 3 and are shipped frozen or in proliferating culture with a confluency of >90 %*. ENDO-Growth Media (MED001) is recommended for culture. Cells have an average additional population doubling levels >18 when cultured. 

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.

*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. 

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.

Product Highlights:
GFP-HUVECs stained with VE-Cadherin and DAPI on an egg white based hydrogel.
GFP-HUVECs stained with VE-Cadherin and DAPI on an egg white based hydrogel.
IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.
IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.
Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.
Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.

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What this product needs to grow and work as described.

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For research use only. Not for diagnostic or clinical use.