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Neuromics
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Primary Antibody · Mouse IgG

Aurora B kinase

Species
Human, Mouse, Rat, Chicken, Bovine, Porcine
Applications
ICC, WB, IHC, IF

Description

Aurora proteins are a family of proteins that play a key role in the regulation of cell division.
HeLa cell cultures were stained with Aurora B kinase antibody (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.
HeLa cell cultures were stained with Aurora B kinase antibody (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.
Left: Western blot analysis of Aurora B in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora B. The Aurora B monoclonal binds strongly to aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with MO22138. This antibody binds specifically to Aurora B. This is also consisted with the immunocytochemical staining of midzones and midbodies on HeLa cells.
Left: Western blot analysis of Aurora B in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora B. The Aurora B monoclonal binds strongly to aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with MO22138. This antibody binds specifically to Aurora B. This is also consisted with the immunocytochemical staining of midzones and midbodies on HeLa cells.

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For research use only. Not for diagnostic or clinical use.