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Neuromics
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Primary Antibody · Mouse IgG

SARS-CoV2 S-Protein ACE2 Binding Domain

Species
Not Applicable
Applications
ICC, WB, IF

Description

In late 2019 a novel infectious disease was discovered in Wuhan, China which was quickly recognized to be caused by a previously unknown RNA coronavirus. The virus was very rapidly isolated, the full RNA sequence determined and put on-line on the 10th of January 2020. The sequence revealed that the virus was most closely related to certain bat coronaviruses and the severe acute respiratory syndrome (SARS) coronavirus. Immediately biotechnology companies and research institutes used the RNA sequence information to generate vaccine candidates. The SARS virus was known to enter and infect human cells by means of the so-called spike or S-protein which binds to the extracellular domain of the angiotensin converting enzyme 2 (ACE2) protein, which is then internalized bringing the virus into the cell. Cryoelectron microscopy and binding studies quickly determined that the S-protein of SARS-CoV2 is structurally similar to to that of the SARS virus and also binds to the ACE2 receptor, albeit with higher affinity than the S-protein of SARS.
Cells were transfected with the DNA encoding the S-protein segment in the pCI-Neo-Mod expression vector. The MO22195 produces clean and specific staining of transfected cells. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.
Cells were transfected with the DNA encoding the S-protein segment in the pCI-Neo-Mod expression vector. The MO22195 produces clean and specific staining of transfected cells. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.
Cells were transfected with DNA encoding the S-protein ACE2 binding site which was inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, which express either the insert only or the insert fused with GFP.
Cells were transfected with DNA encoding the S-protein ACE2 binding site which was inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, which express either the insert only or the insert fused with GFP.

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For research use only. Not for diagnostic or clinical use.