Description

BACE1, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. BACE-1 is a member of the peptidase A1 protein family and is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. In brains of patients with Alzheimer's disease, BACE-1 is detected in reactive astrocytes, suggesting that astrocyte activation may play a role in the development of Alzheimer's disease.

Immunohistochemical detection of BACE-1 in astrocytes in rat cortex. Rat brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to BACE-1 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemical detection of BACE-1 in mouse cerebellum. Mouse brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to BACE-1 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue)

Immunohistochemical detection of BACE1 in reactive astrocytes in formalin fixed Alzheimer's human brain.12 μm paraffin-embedded tissue sections were incubated with rabbit polyclonal antibody to MMP1 at 8 μg/mL overnight at 4°C followed by HRP/AEC detection (red) and counterstaining with hematoxylin (blue).

Description

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