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Neuromics
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Primary Antibody · Rabbit IgG

Transforming Growth Factor (TGF) beta 1, middle region

Species
Human, Mouse
Applications
ICC, WB, IHC, IF

Description

TGF beta 1 (TGFB1) is a protein performing many fiunctions including control of proliferation and differentiation, and regulates the actions of many other growth factors. It is a potent stimulator of osteoblastic bone formation. Inactive TGFB1 is a homodimer consisting of a TGFB1 which is non-covalently linked to a latency-associated peptide (LAP), and the active form of TGFB1 is a homodimer of mature TGFB1 protein.
Immunohistochemical detection of TGF beta 1 in distal convoluted tubules in mouse kidney cortex. Tissue was fixed with 4% formaldehyde and cut into 12 μm thick cryostat sections. Tissue sections were incubated with rabbit polyclonal antibody to TGF beta 1 at 8 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemical detection of TGF beta 1 in distal convoluted tubules in mouse kidney cortex. Tissue was fixed with 4% formaldehyde and cut into 12 μm thick cryostat sections. Tissue sections were incubated with rabbit polyclonal antibody to TGF beta 1 at 8 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemical detection of TGF beta 1 in formalin fixed human prostate. 5 μm paraffin-embedded tissue sections were incubated with rabbit polyclonal antibody to MMP2 at 8 μg/mL overnight at 4°C followed by HRP/AEC detection (red) and counterstaining with hematoxylin (blue).
Immunohistochemical detection of TGF beta 1 in formalin fixed human prostate. 5 μm paraffin-embedded tissue sections were incubated with rabbit polyclonal antibody to MMP2 at 8 μg/mL overnight at 4°C followed by HRP/AEC detection (red) and counterstaining with hematoxylin (blue).
Western blot analysis using RA21028
Western blot analysis using RA21028

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For research use only. Not for diagnostic or clinical use.