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Neuromics
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Primary Antibody · Rabbit IgG

Galectin-3 Rabbit Polyclonal Antibody

Species
Human, Mouse, Rat
Applications
ICC, WB, IF

Description

Galectin 3 is a member of a multigene family of 15 vertebrate lectins all of which share one or in some cases two “carbohydrate recognition domains” (CRD) which bind to β-galactoside, a sugar moiety found on many cell surface proteins. Much evidence suggests that Gal-3 has an important role in the regulation of inflammatory responses and fibrogenesis. In the brain it is expressed in microglia and certain astrocytes. Antibody to this protein can therefore be used to study inflammation within and outside the brain. 
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to galectin-3, RA22131, dilution 1:2,000 in green, and costained with mouse mAb to GFAP dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to galectin-3, RA22131, dilution 1:2,000 in green, and costained with mouse mAb to GFAP dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA.
Western blot analysis of different tissue and cell lysates using rabbit pAb to galectin-3, RA22131, dilution 1:5,000 in green: [1] protein standard, mouse tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] rat cortical neuron-glial primary cell culture lysate, and [7] pig spinal cord. The band at about 30kDa corresponds to the galectin-3 protein.
Western blot analysis of different tissue and cell lysates using rabbit pAb to galectin-3, RA22131, dilution 1:5,000 in green: [1] protein standard, mouse tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] rat cortical neuron-glial primary cell culture lysate, and [7] pig spinal cord. The band at about 30kDa corresponds to the galectin-3 protein.

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For research use only. Not for diagnostic or clinical use.