Sulfo-SMA

Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.

Another major advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.

After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag.

Features

Usage: Membrane protein solubilization & stabilization

Full-Name: Poly(styrene-co-N′,N′-dimethyl(maleimidoethyl)ammonium propane sulfonate) copolymer

Formula Weight: 8,000 g/mol ± 1000 g/mol

Buffer: 50 mM HEPES

pH upon addition of dH2O: 7.5 ± 0.3

Total pH stability: 4-10

State: Lyophilized Powder

Recommended concentration range upon use: 1% to 5%

Solubility: > 10 % in H2O

Mg2+ Tolerance: over 100 mM

Ca2+ Tolerance: over 100 mM

Color: White to slightly yellow

Odor: Odorless

Shipping Temperature: ambient temperature

Long-term Storage (lyophilized copolymer): -20°C for several years

Short-term Storage (dissolved copolymer): 2-8°C for several days