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Neuromics

Protein Purification · Blocking Buffer

BioLock Biotin blocking solution

Cited in 12 papers
Species
Not Applicable

Published with this product (12)

All 12 publications

Description

Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.

Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin® chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.

Activity: >70U/ml

Add at least 1U of BioLock solution per µg of biotin.

BioLock Biotin blocking solution
BioLock Biotin blocking solution

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For research use only. Not for diagnostic or clinical use.