Published with this product (2)
2021PLoS Pathogens, 17(11), e1010052
Flagellin Lysine Methyltransferase FliB Catalyzes a [4Fe-4S] Mediated Methyl Transfer Reaction
Chu Wang, et al
doi:10.1371/journal.ppat.10100522017Toxins, 9(9), 288
Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution
Franziska Tausch, et al
doi:10.3390/toxins9090288
Description
Description
Expression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed recombinant protein will be secreted into the periplasm
Affinity tag
Strep Tactin® affinity tag (Strep tag II ® ) for the purification of recombinant protein. The affinity tag is fused to the C terminus of the recombinant protein.
Bacterial Expression
Expression is induced upon addition of 200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A550= 0.5).
Expression strain
Any E. coli strain. The tet promoter works independently from the genetic background of E. coli.
Resistance
Chloramphenicol. Note: The CamR resistance gene codes for homotetrameric chloramphenicol acetyltransferase (MW of the monomer = 26.6 kDa) which is predominantly expressed in the cytosol of E.coli transformed with this plasmid
Form
5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 ul.
Concentration
250 ng/ul.
Storage
4 °C for frequent usage, 20 °C for long term storage.
Related products
For research use only. Not for diagnostic or clinical use.