Published with this product (3)
2020PCC 6803 is Under Global Metabolic Control by PII Signaling
Phosphoenolpyruvate Carboxylase from the Cyanobacterium Synechocystis sp
Jörg Scholl, et al
doi:10.1111/mmi.145122017Toxins, 9(9), 288
Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution
Franziska Tausch, et al
doi:10.3390/toxins90902882014Journal of Biological Chemistry, 289(40), 27899-27910
Sebastian Dintner, et al
doi:10.1074/jbc.M114.596221
Description
Expression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed recombinant protein will be localized in the cytoplasm.
Affinity tagStrep-Tactin ® affinity tag (Strep-tag II ®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein.
Bacterial Expression Expression is induced upon addition of 200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A550= 0.5). Expression strain Any E. coli strain. The tet promoter works independently from the genetic background of E. coli. ResistanceChloramphenicol.Note: The CamR resistance gene codes for homotetrameric chloramphenicol acetyltransferase (MW of the monomer = 26.6 kDa) which is predominantly expressed in the cytosol of E.colitransformed with this plasmid
Form 5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 ul. Concentration 250 ng/ul. Storage 4 °C for frequent usage, 20 °C for long term storage.
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For research use only. Not for diagnostic or clinical use.