Published with this product (2)
2021Cell, 184(5), 1171-1187.E20
Emma C. Thomson, et al
doi:10.1016/j.cell.2021.01.0372020Scientific Reports, 10, 14243
Sandeep Satapathy, et al
doi:10.1038/s41598-020-70990-3
Description
Replacing:
Advantages of Strep-Tactin®XT 4Flow® vs. Strep-Tactin®XT Superflow®:
Strep-Tactin®XT 4Flow®
Strep-Tactin®XT Superflow®
pH stability
4-10
6-10
Protein binding capacity
High
High
Addressed protein size
Small to large proteins
Small to medium proteins (< 90 kDa)
Yield of low abundant proteins
Excellent
Excellent
Yield of large proteins
5-fold increased
Acceptable
Compatible with challenging proteins
Perfectly suitable
Perfectly suitable
Strep-Tactin®XT 4Flow® high capacity cartridge for one-step purification of recombinant Strep-tag®II and Twin-Strep-tag® fusion proteins with HPLC or FPLC
The cartridges are primarily designed for use with chromatography workstations with 10-32 connections (please note that you do not need adapters for HPLC and Äkta). They can, however, also be operated with other workstations, with syringes or with peristaltic pumps by use of common adapters for cartridges. Since column bodies are pressure resistant for up to 3 bar, the cartridges can be used with a flow restrictor. Cartridges can be connected in series to enlarge capacity.
Dynamic binding capacity: 16 mg protein/ml resin, determined with mCherry-Twin-Strep-tag® (30kDa).
Related products
For research use only. Not for diagnostic or clinical use.