PureCube HiCap StrepTactin MagBeads

PureCube Strep-Tactin® MagBeads is a suspension of 6% cross-linked magnetic agarose beads that is coupled to the Strep-Tactin® protein. They enable protein yields of up to 7 mg protein per ml MagBeads. They were developed for protein purification assays using the Strep-Tactin® using magnetic separation.

Features

Usage: Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins

Specificity: Affinity to Strep-tagged (WSHPQFEK) proteins

Binding capacity: >7 mg/ml

Bead Ligand: Strep-Tactin®

Bead size: 30 μm

Matrix: 6% cross-linked agarose

Filling quantity: Delivered as a 5 % suspension

Chemical stability: 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

Short-term storage: In equilibration buffer (see protocol)

Long-term storage: In 20% ethanol at 4°C