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Neuromics

Protein Purifications

PureCube Ni-INDIGO MagBeads

Applications
Protein Purification

Description

PureCube Ni-INDIGO magnetic beads / MagBeads were developped by Cube Biotech for His-tag protein purification. To create magnetic beads of the highest quality that can tolerate highest concentrations of chelators like EDTA and DTT, which are aften used in mammalian cell culture buffers. The resulting INDIGO ligand has EDTA and DTT stabilities of 20 mM each. That is highly superior to other competing magnetic beads like Ni-NTA.

Features

Usage: Specific binding and purification of 6x His-tagged proteins

Specificity: Affinity to His-tagged proteins

Binding capacity: >100 mg/mL

Bead Ligand: Ni-INDIGO

Bead size: 30 μm

Filling quantity: Delivered as a 25 % suspension

Chelator stability: Stable in buffer containing 20 mM DTT and 20 mM EDTA

pH stability: 2-14

Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

PureCube Ni-INDIGO MagBeads

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For research use only. Not for diagnostic or clinical use.