Description
The HisCube MagBead protein purification MINI Kit is designed to meet the demands of your laboratory for efficient and high-quality small-scale His-tag protein purifications. The included MagBead separator offers a comprehensive and user-friendly solution.
The HisCube INDIGO-Ni MagBead MINI Kit contains PureCube INDIGO Ni-MagBeads.
The major benefit of INDIGO is its tolerance for chelating and reducing agents. While these can be a huge assistance in His-tag purifications, they are not tolerated by conventional His-tag affinity beads. EDTA is a chelator that is often used in eukaryotic cell buffers because it has protease-inhibiting functions. DTT can dissolve protein aggregates that could potentially hinder the access of the purification beads to the His-tagged proteins. However, both chemicals are now usable because of INDIGO. As a result, they are a part of the Kit.
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For research use only. Not for diagnostic or clinical use.