HisCube - Ni-INDIGO His-Tag Protein Purification MIDI Kit

The HisCube His-tag protein purification MINI Kit supplies your laboratory with a convenient method for high-quality small-scale His-tag protein purifications. This kit provides a protocol that is easy to follow.

The HisCube Ni-INDIGO MINI Kit contains PureCube 100 Ni-INDIGO agarose resin and our PureCube 1-Step Batch MINI batch spin tubes.

INDIGO's greatest advantage is its tolerance towards reducing and chelating agents. These can be used to great help in His-tag purifications, but traditional His-tag affinity beads do not tolerate them. EDTA is a chelator that is often used in eukaryotic cell buffers because it has protease-inhibiting functions. DTT can dissolve protein aggregates that could potentially hinder the access of the purification beads to the His-tagged proteins. But thanks to INDIGO both substances can be used now. Therefore they are included in the Kit.