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Neuromics

Human cells · Human Primary CAFs

Human Colorectal Tumor CAFs

Cited in 11 papers
Species
Human
Applications
Cell Assays

Published with this product (11)

All 11 publications

Description

Carcinoma associated fibroblasts (CAFs) have recently been implicated in important aspects of epithelial solid tumor biology such as neoplastic progression, tumor growth, angiogenesis, and metastasis. Human Colorectal CAFs are isolated from human colon tumor tissue. Cells are grown in T75 tissue culture flasks. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1,000,000 cells.

Human Colorectal CAFs can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. Cells can be expanded for 3-5 passages.

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.

Product Highlights:
Colorectal Tumor CAFs in culture. Grown using CAF Growth Media (cat. CAFM03)
Colorectal Tumor CAFs in culture. Grown using CAF Growth Media (cat. CAFM03)
Expression of common markers in patient-derived fibroblasts. Immunofluorescent staining of primary colon cancer fibroblasts (Neuromics, #CAF05), reveals a heterogeneous expression pattern for both αSMA/ACTA2 (abcam #ab7817, 1/200) and FAP (Santa-Cruz Biotechnology #sc-65,398, 1/200), while PDGFRα (abcam #ab61219, 1/200) expression in tested cells remains relatively homogenous.
Expression of common markers in patient-derived fibroblasts. Immunofluorescent staining of primary colon cancer fibroblasts (Neuromics, #CAF05), reveals a heterogeneous expression pattern for both αSMA/ACTA2 (abcam #ab7817, 1/200) and FAP (Santa-Cruz Biotechnology #sc-65,398, 1/200), while PDGFRα (abcam #ab61219, 1/200) expression in tested cells remains relatively homogenous.
Colorectal Tumor CAFs in culture. Grown using CAF Growth Media (cat. CAFM03)
Colorectal Tumor CAFs in culture. Grown using CAF Growth Media (cat. CAFM03)

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For research use only. Not for diagnostic or clinical use.