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Neuromics
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Primary Antibody · Chicken IgY

Nestin

Species
Human, Mouse, Rat
Applications
WB, IHC, IF

Description

Nestin belongs to the class IV intermediate filament protein family. Nestin is expressed in radial glia and other dividing cells in the developing CNS, PNS and muscles. Nestin is also found to be expressed in brain tumors, particularly gliomas. Nestin is also a marker of stem cells in the pancreas and heart. The nestin amino acid sequence is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result, antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody works well on both human and rodent cells and tissues.
Immunofluorescent analysis of rat embryonic (E18) brain stained with chicken Nesting (CH22123), dilution 1:2,000 in red. Nuclear DNA is stained blue with Hoechst.
Immunofluorescent analysis of rat embryonic (E18) brain stained with chicken Nesting (CH22123), dilution 1:2,000 in red. Nuclear DNA is stained blue with Hoechst.
Western blot analysis of different cell lysates using rabbit pAB to nestin, CH22123, dilution 1:3,000 in green: [1] protein standard, [2] rat cortical neuron-glial cell culture , and [3] SH-SY5Y cells. High molecular weight band corresponds to nestin protein detected only in human cells.
Western blot analysis of different cell lysates using rabbit pAB to nestin, CH22123, dilution 1:3,000 in green: [1] protein standard, [2] rat cortical neuron-glial cell culture , and [3] SH-SY5Y cells. High molecular weight band corresponds to nestin protein detected only in human cells.

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For research use only. Not for diagnostic or clinical use.