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Neuromics
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Primary Antibody · Chicken IgY

Ki67

Species
Human, Mouse, Rat
Applications
ICC, WB, IF

Description

The Ki67 protein was first discovered when researchers attempted to generate cancer cell specific monoclonal antibodies by injecting mice with nuclear preparations from Hodgkin’s lymphoma cells. They obtained a monoclonal antibody which recognized two large proteins of apparent molecular weight 345kDa and 395kDa. The presence of the Ki67 protein is frequently used as an indicator of cell proliferation and its level of expression is one of the most reliable biomarkers of proliferative status of cancer cells. Much research shows a correlation between Ki67 protein level and prognosis in cancer patients, when high Ki67 levels being associated with poorer outcomes.

Product Highlights:
Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki67, CH22135, dilution 1:2,000 in red, and mouse mAb to β-tubulin in green. The blue is DAPI staining of nuclear DNA. The CPCA-Ki67 antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state.
Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki67, CH22135, dilution 1:2,000 in red, and mouse mAb to β-tubulin in green. The blue is DAPI staining of nuclear DNA. The CPCA-Ki67 antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state.
Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, CH22135, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa.
Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, CH22135, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa.

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For research use only. Not for diagnostic or clinical use.