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Neuromics
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Primary Antibody · Mouse IgG

Muscleblind-like Protein

Species
Human, Mouse, Rat, Chicken, Bovine, Porcine
Applications
ICC, WB, IHC, IF

Description

Muscleblind-like Protein (MBNL1) mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5-YGCU(U/G)Y-3consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Diseases associated with MBNL1 include myotonic dystrophy and myotonic dystrophy 1. Among its related pathways are Adipogenesis. GO annotations related to this gene include poly(A) RNA binding and double-stranded RNA binding
HeLa cells were stained with monoclonal antibody Muscleblind-like protein (MO22147) at 1: 1,000. Muscleblind-like protein 1 is present in the nucleus of HeLa cells (red). Co-staining is with our rabbit polyclonal antibody against vimentin (RA22124, in red) reveals cytoplasmic intermediate filaments. Blue is a DNA stain which largely colocalizes with the MBNL1 protein in the nucleus.
HeLa cells were stained with monoclonal antibody Muscleblind-like protein (MO22147) at 1: 1,000. Muscleblind-like protein 1 is present in the nucleus of HeLa cells (red). Co-staining is with our rabbit polyclonal antibody against vimentin (RA22124, in red) reveals cytoplasmic intermediate filaments. Blue is a DNA stain which largely colocalizes with the MBNL1 protein in the nucleus.
Western blot analysis of MO22147. Blot of HL60 cell lysate was probed with MO22147 at dilution 1:1,000. The MO22147 monoclonal binds Muscleblind-like protein 1 at ~40kDa, as expected.
Western blot analysis of MO22147. Blot of HL60 cell lysate was probed with MO22147 at dilution 1:1,000. The MO22147 monoclonal binds Muscleblind-like protein 1 at ~40kDa, as expected.

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For research use only. Not for diagnostic or clinical use.