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Neuromics
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Primary Antibody · Mouse IgG

Neurofilament NF-L

Species
Human, Mouse, Rat, Bovine, Porcine, Equine
Applications
ICC, WB, IHC, IF

Description

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins, but in certain cell types and during development α-internexin, peripherin, nestin and vimentin may be included also. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and many other.

The antibody was made against a preparation of recombinant full length human NF-L protein expressed in and purified from E. coli. This antibody is known to bind NF-L from a variety of species including human, rat and mouse, and the epitope is 100% conserved in all mammalian NF-L sequences, so this antibody will have wide applicability.

Product Highlights:
Immunofluorescent analysis of cow cerebellum section stained with mouse mAb to NF-L, MO22201, dilution 1:2,000 in green, and costained with chicken pAb to VLP1 in red. Blue is Hoechst staining of nuclear DNA. MO22201 labels dendrites and axons of neuronal cells in the granular layer (lower left) and prominent basket cell axons surrounding the large Purkinje neurons. The VLP1 antibody reveals protein expressed in granule cells and in synapses of the molecular layer of the cerebellum.
Immunofluorescent analysis of cow cerebellum section stained with mouse mAb to NF-L, MO22201, dilution 1:2,000 in green, and costained with chicken pAb to VLP1 in red. Blue is Hoechst staining of nuclear DNA. MO22201 labels dendrites and axons of neuronal cells in the granular layer (lower left) and prominent basket cell axons surrounding the large Purkinje neurons. The VLP1 antibody reveals protein expressed in granule cells and in synapses of the molecular layer of the cerebellum.
Western blot analysis of different tissue lysates using mouse mAb to NF-L, MO22201, dilution 1:20,000 in green: [1] protein standard, [2] rat brain , [3] mouse brain and [4] cow cerebelum. Strong band at about 68-70kDa corresponds to NF-L protein, with the cow protein appearing slightly larger in molecular size as expected. Low molecular weight bands detected in cow brain sample are likely post mortem proteolytic forms of NF-L.
Western blot analysis of different tissue lysates using mouse mAb to NF-L, MO22201, dilution 1:20,000 in green: [1] protein standard, [2] rat brain , [3] mouse brain and [4] cow cerebelum. Strong band at about 68-70kDa corresponds to NF-L protein, with the cow protein appearing slightly larger in molecular size as expected. Low molecular weight bands detected in cow brain sample are likely post mortem proteolytic forms of NF-L.

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For research use only. Not for diagnostic or clinical use.