Published with this product (3)
2024Nature Communications, 15
A Specific Folate Activates Serotonergic Neurons to Control C. elegans Behavior
Ria S. Peesapati, Brianna L. Austin-Byler, Fathima Zahra Nawaz, Jonathan B. Stevenson, Stanelle A. Mais, Rabia N. Kaya, Michael G. Hassan, Nabraj Khanal, Alexandra C. Wells, Deena Ghiai, Anish K. Garikapati, Jacob Selhub, and Edward T. Kipreos
doi:10.1038/s41467-024-52738-z2023Molecular Metabolism, 101706
Katharina Wissmiller, Sara Bilekova, Andras Franko, Stefan Z. Lutz, Miriam Katsburg, Sebastian Gulde, Natalia S. Pellegata, Arnulf Stenzl, Martin Heni, Lucia Berti, Hans-Ulrich Häring, and Heiko Lickert
doi:10.1016/j.molmet.2023.1017062021Nature Metabolism, 3:1202–1216
Diet-Induced Alteration of Intestinal Stem Cell Function Underlies Obesity and Prediabetes in Mice
Alexandra Aliluev, Sophie Tritschler, Michael Sterr, Lena Oppenländer, Julia Hinterdobler, Tobias Greisle, Martin Irmler, Johannes Beckers, Na Sun, Axel Walch, Kerstin Stemmer, Alida Kindt, Jan Krumsiek, Matthias H. Tschöp, Malte D. Luecken, Fabian J. Theis, Heiko Lickert, and Anika Böttcher
doi:10.1038/s42255-021-00458-9
Description
The histochemical antibody for serotonin is generated in a rabbit against serotonin coupled to bovine serum albumin with paraformaldehyde.
The antibody produces a 4+ indirect immunofluorescence staining at a 1/1,000 - 1/2,000 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/20,000 - 1/40,000 dilution in rat hypothalamus, raphe nuclei and spinal cord. Staining is completely eliminated by pretreatment of the diluted antibody with 25 µg of serotonin/BSA.
Cross reactivity of Serotonin antisera was examined. With 5 µg, 10 µg and 25 µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.
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For research use only. Not for diagnostic or clinical use.