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Neuromics
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Primary Antibody · Rabbit IgG

CasΦ/Cas12j

Species
Not Applicable
Applications
ICC, WB, IF

Description

There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. One of these enzymes is referred to as CasΦ and also known as Cas-12j. The use of such smaller enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.

RA22145 was made against a recombinant construct of the full sequence of CasΦ-2. The antibody works well on western blots of crude homogenates of HEK293 cells transfected with the Cas%Phi;-2 DNA, cleanly producing the appropriate sized band. In addition such transfected cells show clean and strong cytoplasmic staining by immunofluorescence staining with this antibody.

Product Highlights:
Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding full length CasΦ protein and stained with rabbit pAb against CasΦ, RA22145, in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals cytoplasmic expression of the CasΦ protein only in transfected cells.
Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding full length CasΦ protein and stained with rabbit pAb against CasΦ, RA22145, in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals cytoplasmic expression of the CasΦ protein only in transfected cells.
Western blot analysis of HEK293 cell lysates using rabbit pAb to CasΦ protein, RA22145, dilution 1:1,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to the expected GFP-CasΦ fusion protein.
Western blot analysis of HEK293 cell lysates using rabbit pAb to CasΦ protein, RA22145, dilution 1:1,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to the expected GFP-CasΦ fusion protein.

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For research use only. Not for diagnostic or clinical use.