Description
Strep-Tactin® 4Flow® high capacity is a resin for purification of strep-tagged proteins via affinity chromatography. The product is optimized for column-based use and suitable for HPLC/FPLC. It consists out of cross-linked agarose beads that are coupled to Strep-Tactin®, which is a streptavidin variant. Strep-Tactin® binds to proteins that were fused to a Strep-tag®II or a Twin-Strep-tag®, enabling a straightforward purification of tagged proteins from mixed samples, reaching a purity of >95%. Due to the targeted elution, tedious titration steps are not necessary. In addition, regeneration allows for multiple use of the resin. This resin is an alternative for proteins that are difficult to elute from our Strep-Tactin®XT 4Flow® resins.
Replacing:
Name
Catalog Number
Strep-Tactin® Sepharose® Resin
2-1201-002
Strep-Tactin® Superflow® High Capacity Resin
2-1208-002
Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose® and Strep-Tactin® Superflow®:
Strep-Tactin® Sepharose®
Strep-Tactin® Superflow®
Strep-Tactin® 4Flow®
Bead structure
4% agarose, crosslinked
6% agarose, crosslinked
4% agarose, highly crosslinked
Bead size
45-165 um
60-160 um
50-150 um, spherical
Exclusion limit
~3 x 10^7 Da
6 x 10^6 Da
3 x 10^7 Da
Recommended flow rate
gravity flow
0.5-1 ml/min
0.5-1 ml/min
pH range for protein binding
7-8
7-8
7-8
Max pressue
gravity flow
9.6 bar
3.5 bar
Eluent
desthiobiotin
desthiobiotin
desthiobiotin
Regeneration buffer
Buffer R
Buffer R
100 mM NaOH
Related products
- 10x Buffer BXT; Strep-Tactin®XT Elution Buffer
- Avidin
- BioLock Biotin blocking solution
- Biotin
- Buffer XT-R
- Desthiobiotin
- Empty Spin Columns
- GFP-Strep-tag control protein
- GFP-Twin-Strep-tag control protein
- Magnetic Separator
- MagStrep "type3" Strep-Tactin® beads
- MagStrep Strep-Tactin®XT Beads
For research use only. Not for diagnostic or clinical use.