Description
Strep-Tactin® 4Flow® high capacity FPLC columns are ready-to-use columns for affinity chromatography. They are suitable for automated protein purification with HPLC/FPLC devices such as Äkta® systems (Cytiva). The FPLC columns contain a cross-linked agarose coupled to the ligand Strep -Tactin®, a streptavidin variant that binds to Strep-tag®II or Twin-Strep-tag® fusion proteins. This protein purification system is characterized by a high target purity of >95% and a stable performance even after several regeneration cycles. These columns are the perfect alternative for proteins that are difficult to elute from Strep-Tactin®XT 4Flow® and Strep-Tactin®XT 4Flow® high capacity FPLC columns.
Replacing:
Name
Catalog Number
Strep-Tactin® Superflow® High Capacity FPLC Column
2-1239-001
Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose® and Strep-Tactin® Superflow®:
Strep-Tactin® Sepharose®
Strep-Tactin® Superflow®
Strep-Tactin® 4Flow®
Bead structure
4% agarose, crosslinked
6% agarose, crosslinked
4% agarose, highly crosslinked
Bead size
45-165 um
60-160 um
50-150 um, spherical
Exclusion limit
~3 x 10^7 Da
6 x 10^6 Da
3 x 10^7 Da
Recommended flow rate
gravity flow
0.5-1 ml/min
0.5-1 ml/min
pH range for protein binding
7-8
7-8
7-8
Max pressue
gravity flow
9.6 bar
3.5 bar
Eluent
desthiobiotin
desthiobiotin
desthiobiotin
Regeneration buffer
Buffer R
Buffer R
100 mM NaOH
Related products
- 10x Buffer BXT; Strep-Tactin®XT Elution Buffer
- Avidin
- BioLock Biotin blocking solution
- Biotin
- Buffer XT-R
- Desthiobiotin
- Empty Spin Columns
- GFP-Strep-tag control protein
- GFP-Twin-Strep-tag control protein
- Magnetic Separator
- MagStrep "type3" Strep-Tactin® beads
- MagStrep Strep-Tactin®XT Beads
For research use only. Not for diagnostic or clinical use.