MSP1E3D1-His, Lyophilized Protein

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1E3D1 (His-tagged) creates nanodiscs of 12-14 nm in diameter. This product contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.

This product is available with different species as its background. This is important for e.g. for secondary antibodies of a western blot.

Features

Purity: > 90% (Determined by SDS-PAGE)

Number of amino acids: 277

Molecular Mass: 32,600 Da

Extinction coefficient (in water) ε280: 29,400 M-1cm-1

Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Affinity tag: N-Terminal His-tag

Shipping Temperature: Room Temperature

Storage of lyophilized protein: -20°C for several months

Storage of reconstituted protein: 2-8°C for several days