Mouse MSP2N2-His, Lyophilized Protein

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP2N2 (His-tagged) creates nanodiscs of ~17 nm in diameter.

Note that this products contains a N-terminal His-tag. Keep that in mind when chosing the affinity tag for your membrane protein.

Features

Purity: > 90% (Determined by SDS-PAGE)

Number of amino acids: 388

Molecular Mass: 45,526 Da

Extinction coefficient (in water) ε280: 39,880 M-1cm-1

Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Affinity tag: N-Terminal His-tag

Shipping Temperature: Room Temperature

Storage of lyophilized protein: -20°C for several months

Storage of reconstituted protein: 2-8°C for several days