MSP1D1-D73C-His, Lyophilized Protein

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 D73C creates nanodiscs of 9-10 nm in diameter.

This product contains an additional point mutation, D73C. This additional cystein can be used to further modify the MSP protein and this the nanodisc. And example for such a modification would be the addition of a biotin-tag.

Feature

Purity: > 90% (Determined by SDS-PAGE)

Origin: Human

Number of Amino Acids: 217

Molecular Mass: 25,298 Da

Extinction coefficient (in water) ε280: 25,440 M-1cm-1

Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Affinity tag: N-Terminal His-tag

Shipping Temperature: Room Temperature

Storage of lyophilized protein: -20°C for several months

Storage of reconstituted protein: 2-8°C for several days