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Neuromics
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Primary Antibody · Goat IgG

mCherry

Species
Not Applicable
Applications
ICC, WB, IHC, IF

Description

The mCherry protein is engineered from a fluorescent protein originally isolated from a coral and is widely used as a tracer in transfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from disc corals of the genus Discosoma.

This antibody was made against full length recombinant mCherry protein expressed in and purified from E. coli. The antibody recognizes mCherry and tdTomato on western blots, in appropriate cells and sections, and does not react with GFP. This antibody can be used to verify the size of fusion constructs by western blotting, and to amplify the endogenous fluorescence of mCherry in transfected cells.

Product Highlights:
Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with goat pAb to mCherry, GT22108, dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The GPCA-mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.
Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with goat pAb to mCherry, GT22108, dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The GPCA-mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.
Western blot analysis of HEK293 cell lysates using goat pAb to mCherry, GT22108, dilution 1:1,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain.
Western blot analysis of HEK293 cell lysates using goat pAb to mCherry, GT22108, dilution 1:1,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain.

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For research use only. Not for diagnostic or clinical use.