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Neuromics
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Primary Antibody · Chicken IgY

Green Fluorescent Protein

Species
Not Applicable
Applications
WB, IHC, IF

Description

GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with chicken antibody to GFP, CH22124, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22124 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.
Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with chicken antibody to GFP, CH22124, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22124 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.
Western blot analysis of HEK293 cell lysates using goat pAb to GFP, GPCA-GFP, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct.
Western blot analysis of HEK293 cell lysates using goat pAb to GFP, GPCA-GFP, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct.

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For research use only. Not for diagnostic or clinical use.