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Neuromics
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Human cells · Primary Cells

Human Retinal Microvascular Endothelial Cells

Species
Human
Applications
Cell Assays

Description

HRMECs were initiated by elutriation from dissociated normal human retinal tissue. Passage 1 cells are ship in proliferating culture with a confluency of >90 %*. ENDO-Growth medium is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Characterization of the cells

  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence
HRMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. As the cells are shipped live, extreme weather (including extreme cold or heat) can be a factor if we are able to ship or not. 

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.
Effect of HG (high glucose) and pro-inflammatory cytokines on connexin43 expression in HRMECs. Immunohistochemical data showing connexin43 expression in (A) normal medium; (B) HG (25 mM); (C) pro-inflammatory cytokines (IL-1β and TNF-α 10 ng/mL each); and (D) a combination of HG and pro-inflammatory cytokines inducing a change in cell morphology with signs of cell swelling, possibly owing to hemichannel opening (indicated by white arrows).
Effect of HG (high glucose) and pro-inflammatory cytokines on connexin43 expression in HRMECs. Immunohistochemical data showing connexin43 expression in (A) normal medium; (B) HG (25 mM); (C) pro-inflammatory cytokines (IL-1β and TNF-α 10 ng/mL each); and (D) a combination of HG and pro-inflammatory cytokines inducing a change in cell morphology with signs of cell swelling, possibly owing to hemichannel opening (indicated by white arrows).
Retinal Microvascular Endothelial Cells in culture.
Retinal Microvascular Endothelial Cells in culture.

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For research use only. Not for diagnostic or clinical use.