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Neuromics
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Primary Antibody · Mouse IgM

Green Fluorescent Protein

Species
Not Applicable
Applications
ICC, WB, IHC, IF

Description

GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.
Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.
Western blot analysis of HEK293 cell lysates using mouse mAb to GFP, MO22184, in green, dilution 1:1,000: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry.
Western blot analysis of HEK293 cell lysates using mouse mAb to GFP, MO22184, in green, dilution 1:1,000: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry.

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For research use only. Not for diagnostic or clinical use.