Description
HLIMECs were initiated by elutriation from dissociated normal large intestine tissue. Passage 1 cells are ship in proliferating culture with a confluency of >90 %. ENDO-Grow Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.
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Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
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Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
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Cytoplasmic PECAM1 >95% positive by immunofluorescence
HLIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. As the cells are shipped live, extreme weather (including extreme cold or heat) can be a factor if we are able to ship or not.
Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.
You'll also need
What this product needs to grow and work as described.
- 1X Phosphate Buffer SolutionPBS300 · 100 ml$85.20
- AlphaBioCoat Precoated 100 mm DishesAC001-100MM · Pack of 10$402.00
- AlphaBioCoat Precoated 12-Well PlatesAC001-12 · Pack of 10$352.80
- AlphaBioCoat Precoated 150 mm DishesAC001-150MM · Pack of 10$445.20
- AlphaBioCoat Precoated 24-Well PlatesAC001-24 · Pack of 10$390.00
- AlphaBioCoat Precoated 35 mm DishesAC001-35MM · Pack of 10$205.20
- AlphaBioCoat Precoated 48-Well PlatesAC001-48 · Pack of 10$562.80
- AlphaBioCoat Precoated 6-Well PlatesAC001-6 · Pack of 10$333.60
- AlphaBioCoat Precoated 60 mm DishesAC001-60MM · Pack of 10$279.60
- AlphaBioCoat Precoated 96-Well PlatesAC001-96 · Pack of 10$538.80
- AlphaBioCoat Precoated T-175 FlasksAC001-T175 · Pack of 10$688.80
- AlphaBioCoat Precoated T-25 FlasksAC001-T25 · Pack of 10$286.80
- AlphaBioCoat Precoated T-75 FlasksAC001-T75 · Pack of 10$682.80
- AlphaBioCoat SolutionAC001 · 20 ml$243.60
- Cas9 Endothelial Basal MediaMED005 · 500 ml$325.00
- Cas9 Endothelial Growth MediaMED004 · 500 ml$400.00
- Cell Detachment SolutionADF001 · 50 ml$70.80
- ENDO Basal MediaMED002 · 500 ml$172.80
- ENDO-Growth MediaMED001 · 500 ml$344.40
- ENDO-Growth Media KitEGK001 · 500 ml$344.40
- ENDO-Growth SupplementsEGS001 · 25 ml$214.80
- Endothelial Cryopreserve MediaFM300 · 10 ml$229.20
- Epithelial Cryopreserve MediaFM301 · 20 ml$288.00
- Human Epithelial Cell Basal MediumECM002 · 500 ml$194.40
- Human Epithelial Cell MediumECM001 · 500 mL$301.20
- Smooth Coat SolutionSC300 · 20 ml$229.20
- Universal Tube Formation MediaMED003 · 500 ml$615.00
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For research use only. Not for diagnostic or clinical use.