Ionomycin Calcium

Highly selective nonfluorescent calcium (Ca2+) ionophore [1]. In human neutrophils it induces a rapid rise in cytosolic Ca2+ which is due to both release from cytosolic Ca2+ stores as well as Ca2+ influx [2]. It activates (2 M) and primes (20-200 nM) neutrophil NADPH oxidase [2]. Down regulates beta-catenin/Tcf signaling in a colon cancer cell line via suppressing the binding of Tcf to its specific DNA-binding site [3]. In rat hepatoma cells, sub-lethal ionomycin activates the stress response by activating SAPK/JNK and HSF/HSE interaction leading to upregulation of HSP70 biosynthesis [4].

References:

1) RF Kaufmann et al. J. Biol. Chem. 1980 255:2735

2) DJ Elzi et al. Am. J. Physiol. Cell Physiol. 2001 281:C350

3) CH Park et al. Carcinogenesis 2005 26:1929

4) AS Sreedhar and UK Srinivas J. Cell Biochem. 2002 86:154