Trichostatin A

Potent and selective histone deacetylase (HDAC) inhibitor (Ki = 3.4 nM). Induces reversion of ras transformed cells to normal morphology [1]. Trichostatin A induces dedifferentiation of primordial germ cells into embryonic germ cells [2]. Cell permeable and active in vivo [3]. Decreased global chromatin condensation increasing gene-editing efficiency 2-4X [4]. Part of the CRISPY mix for increasing precise gene editing [5].

References:

1) Yoshida et al. (1990), Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A ; J. Biol. Chem., 265 17174

2) Futamura et al. (1995), Trichostatin A inhibits both ras-induced neurite outgrowth of PC12 cells and morphological transformation of NIH3T3 cells; Oncogene, 10 1119

3) Durcova-Hills et al. (2008), Reprogramming Primordial Germ Cells into Pluripotent Stem Cells; PLoS-One, 3 e3531

4) Molugu et al. (2023), Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells; CRISPR J., 6 473

5) Riesenberg and Maricic (2018), Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells; Nat. Commun., 9 2165